Cellulase Activity Assay Kit (Fluorometric)
The Californer/10129134

MILPITAS, Calif. - Californer -- Cellulases are enzymes that catalyze the decomposition of cellulose molecule into monosaccharides such as beta-glucose, or shorter polysaccharides and oligosaccharides. They are produced by fungi, bacteria, and protozoans. Cellulase is a complex of three different enzymes including β-1,4-endoglucanase, cellobiohydrolase, and β-glucosidase.

Cellulose is a major component of the plant cell wall and most mammals have a very limited ability to digest cellulose by themselves. So cellulose breakdown by fungal cellulases make a major constituent of plants available for consumption. Most fungal cellulases have a two-domain structure, a catalytic domain and a cellulose binding domain. Cellulase is used for commercial food processing in coffee. Additionally, cellulases are widely used in textile industry, laundry detergents, pulp and paper industry, pharmaceutical applications etc.

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BioVision's Cellulase Activity Assay Kit provides a facile, rapid way to measure cellulase activity in various biological samples. In this kit, cellulase cleaves a synthetic substrate and releases the fluorophore, which can be easily quantified at Ex/Em = 365/450 nm. The substrate is specific for cellulase and can differentiate its activity from that of 1,3-β-D-glucanase. The assay is simple, sensitive and can detect cellulase activity lower than 500 nU in samples.

Figures. a. 4-MU Standard Curve, results from multiple experiments. b. Measurement of purified cellulase (2.5 ng) and 1,3-β-D-glucanase (2.5 ng) activities using BioVision's proprietary substrate. The kit can efficiently distinguish the cellulase activity from 1,3-β-D-glucanase. c). Cellulase activity in barley seeds (5 mg) and mushrooms (40 μg protein). All assays were performed following kit protocol.

For more information on this Assay kit, Cat# K2080, Visit: https://bit.ly/3d6rAIS