On-Column DNase Digestion Assay Kit
The Californer/10120404

MILPITAS, Calif. - Californer -- BioVision's On-Column DNase Digestion Kit provides the user a quick and efficient way to remove contaminating genomic DNA from RNA preparations. The kit is designed to be used in conjunction with BioVision's Mammalian RNA Isolation Kit (Cat# K2065) or One Prep DNA/RNA Purification Kit (Cat# K2058) to clean-up or eliminate DNA from purified RNA or from enzymatic reactions. The purified RNA is suitable for numerous downstream applications including RT-PCR. Additionally, the digestion protocol can be used with other commercially available RNA extraction kits

Figure 1: 5 μg Jurkat cell RNA mixed with Hind III-digested λ DNA was purified. 1. RNA without DNase treatment. 2. DNase treatment prior to the RNA Clean-Up Protocol. 3. RNA Clean-Up Protocol with optional On-Column DNA digestion step. RM: Riboruler marker. All

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samples were incubated at RT for 30 min for DNase treatment. 10 μl was loaded per lane.

Figure 2: RNA was purified from Jurkat cells using BV Cat# K2065. Lanes 1 & 3. RNA without DNase treatment. Lanes 2 & 4. RNA with DNase treatment. RM: Riboruler marker. All samples were incubated at RT for 15 min for DNase treatment. 10 μl of elution was loaded per lane.

For more information on this Assay kit Cat# K2066, Visit: https://bit.ly/3qj09iS