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A plate-based fluorometric assay designed to screen, study and characterize potential inhibitors of CD38 hydrolase activity.
MILPITAS, Calif. - Californer -- Cluster of differentiation 38 (CD38), also known as cyclic ADP ribose hydrolase is a type II transmembrane glycoprotein that can function either as a receptor or as an enzyme. It is found on the surface of many immune cells, including plasma B cells, natural killer cells, CD4+, CD8+ etc. It is a multifunctional enzyme involved in cell-adhesion, calcium signaling and Nicotinamide Adenine Dinucleotide (NAD+) metabolism. The hydrolase activity of CD38 helps maintain the appropriate levels of NAD+ for all NAD+ dependent metabolic processes to occur. Elevated levels of CD38 are associated with aging, obesity, diabetes, heart disease, asthma, inflammation and tumorigenesis etc.
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BioVision's CD38 (Hydrolase) Inhibitor Screening Kit is a plate-based fluorometric assay designed to screen, study and characterize potential inhibitors of CD38 hydrolase activity. The assay utilizes a selective CD38 substrate to generate a fluorescent signal measured at Ex/Em = 300/410 nm. In the presence of potential CD38 hydrolase inhibitors, the fluorescent signal is reduced. The assay is quick, easy, and sensitive for high-throughput screening of CD38 inhibitors. Additionally, the kit includes a CD38 Inhibitor as a control inhibitor.
Figure: Inhibition of CD38 activity by Apigenin. IC50 was calculated to be 6.1 ± 0.1 μM. Assay was performed following the kit protocol.
For more information on this Assay Kit Cat# K2086, Visit: https://bit.ly/3ofloCb
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BioVision's CD38 (Hydrolase) Inhibitor Screening Kit is a plate-based fluorometric assay designed to screen, study and characterize potential inhibitors of CD38 hydrolase activity. The assay utilizes a selective CD38 substrate to generate a fluorescent signal measured at Ex/Em = 300/410 nm. In the presence of potential CD38 hydrolase inhibitors, the fluorescent signal is reduced. The assay is quick, easy, and sensitive for high-throughput screening of CD38 inhibitors. Additionally, the kit includes a CD38 Inhibitor as a control inhibitor.
Figure: Inhibition of CD38 activity by Apigenin. IC50 was calculated to be 6.1 ± 0.1 μM. Assay was performed following the kit protocol.
For more information on this Assay Kit Cat# K2086, Visit: https://bit.ly/3ofloCb
Source: BioVision, Inc.
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