Trending...
- California: Governor Newsom signs legislation 7.3.25
- California: Governor Newsom proclaims Independence Day 2025
- New Release: 'The Invisible Alternative' Unveiled by Atrisk Corporation, Resilient
A simple, plate-based colorimetric assay for measuring ATP synthase in isolated mitochondria
MILPITAS, Calif. - Californer -- Mitochondrial ATP synthase (EC 3.6.3.14), also known as mitochondrial complex V or F1F0-ATP synthase is a multi-subunit membrane-bound enzyme of mitochondria that utilizes the proton electrochemical gradient generated by the respiratory chain for ATP synthesis. It consists of two domains, an inner membrane embedded domain (F0) and a membrane extrinsic domain (F1), which is also the catalytic core of the enzyme.
In addition to its critical role in ATP synthesis, ATP synthase regulates the flux of oxidative phosphorylation, mitochondrial signaling by reactive oxygen species, cell death etc. Defects in ATP synthase have been associated with human diseases including neuromuscular disorders, heart failure etc. Thus, ATP synthase may be used as a therapeutic drug target in the treatment of many diseases.
BioVision's Mitochondrial ATP Synthase Activity Assay Kit is a simple, plate-based colorimetric assay for measuring ATP synthase in isolated mitochondrial samples. In this assay, ATP Synthase hydrolyzes ATP to ADP. In the subsequent steps, ADP together with ATP synthase converter and enzyme mix oxidizes NADH to NAD+, which is monitored via a decrease in absorbance at 340 nm. A specific ATP synthase inhibitor, oligomycin is also included in the kit.
More on The Californer
Sample specific ATP synthase activity is obtained by measuring the sample ATP synthase activity in the presence and absence of oligomycin. The assay provides a simple, rapid, and sensitive method to measure ATP synthase activity in isolated mitochondria.
Figures. (A) ATP Synthase activity in purified mitochondria with and without Oligomycin.
(B) ATP Synthase activity using varying concentrations of Jurkat cell mitochondria (isolated using BioVision Cat# K288-50). (C) ATP Synthase activity in mitochondria isolated from Jurkat cells, HepG2 cells, rat heart (isolated using BioVision Cat# (K288-50) and bovine heart mitochondria (obtained commercially) with and without Oligomycin. Note: Net ATP Synthase activity in sample = Activity in reaction without Oligomycin – Activity in reaction with Oligomycin. All assays were performed following kit protocol.
Storage Conditions Multiple Temperatures
USAGE For Research Use Only! Not For Use in Humans.
For more information on this Assay Kit Cat# K2112 https://bit.ly/30Lsx5H
In addition to its critical role in ATP synthesis, ATP synthase regulates the flux of oxidative phosphorylation, mitochondrial signaling by reactive oxygen species, cell death etc. Defects in ATP synthase have been associated with human diseases including neuromuscular disorders, heart failure etc. Thus, ATP synthase may be used as a therapeutic drug target in the treatment of many diseases.
BioVision's Mitochondrial ATP Synthase Activity Assay Kit is a simple, plate-based colorimetric assay for measuring ATP synthase in isolated mitochondrial samples. In this assay, ATP Synthase hydrolyzes ATP to ADP. In the subsequent steps, ADP together with ATP synthase converter and enzyme mix oxidizes NADH to NAD+, which is monitored via a decrease in absorbance at 340 nm. A specific ATP synthase inhibitor, oligomycin is also included in the kit.
More on The Californer
- Stuck Doing Math or Figuring Out Life's Numbers? Calculator.now Makes It Stupidly Simple
- Cal State LA secures funding for two artificial intelligence projects from CSU
- Colbert Packaging Announces WBENC Recognition
- New Mobile Car Detailing Platform Connects Drivers with On-Demand Local Pros
- Over the past three months, California seized $476 million worth of unlicensed cannabis products
Sample specific ATP synthase activity is obtained by measuring the sample ATP synthase activity in the presence and absence of oligomycin. The assay provides a simple, rapid, and sensitive method to measure ATP synthase activity in isolated mitochondria.
Figures. (A) ATP Synthase activity in purified mitochondria with and without Oligomycin.
(B) ATP Synthase activity using varying concentrations of Jurkat cell mitochondria (isolated using BioVision Cat# K288-50). (C) ATP Synthase activity in mitochondria isolated from Jurkat cells, HepG2 cells, rat heart (isolated using BioVision Cat# (K288-50) and bovine heart mitochondria (obtained commercially) with and without Oligomycin. Note: Net ATP Synthase activity in sample = Activity in reaction without Oligomycin – Activity in reaction with Oligomycin. All assays were performed following kit protocol.
Storage Conditions Multiple Temperatures
USAGE For Research Use Only! Not For Use in Humans.
For more information on this Assay Kit Cat# K2112 https://bit.ly/30Lsx5H
Source: BioVision Inc
0 Comments
Latest on The Californer
- Hamilton Zanze Sponsors the Acquisition of Two Garden-Style Communities in Reno Area
- Meet a Scientologist Captures Greece's Timeless Beauty with Videographer Lambros Malamas
- Vinnetwork Unveils Decentralized AI Platform with Vinnetwork(VIN) Token to Challenge Tech Giants' Data Monopoly
- Moovs Launches Advanced Contact Center Solution for Large-Scale Transportation Operations
- Centennial Flyers to Become Colorado's First Launch Customer for All-Electric B23 Energic Aircraft
- Second Annual Artists' Rights Advocate Award to Be Presented at The Comedy Store on July 17th
- Pyro Marketing Opens New Digital Marketing Company in Saint Petersburg to Power Growth for Fitness and Ecommerce Brands
- Dr. John Salerno of Salerno Wellness Introduces Their New Full Body Capsule for Advanced LED Light Therapy Patient Treatments
- Ship Overseas Inc. Expands Specialized Shipping Services
- Governor Newsom announces additional deployment of California resources to support New Mexico following Texas and Oregon disaster response efforts
- Wise Business Plans® Earns Top Ratings as Leading Authority in Professional Business Plans
- Coming Up On this Week's Podcast "Financial Freedom with Tom Hegna" Kari Ellis
- Voices for Humanity Builds a Better Future for Ghana with Richmond Atta-Williams
- Haunted Doll House at The Gregangelo Museum
- Mystical Insights at the Gregangelo Museum
- Ben Pouladian, CEO of BEP Holdings, to Join ISPE Los Angeles Biomanufacturing Panel
- Raksmart: Promoting Security in Global Hosting Service
- $14M Expansion Deal with Famed David Lloyd Highlights Rebrand of Sports, Entertainment and Gaming Innovation by AI Driven, Online Fan Engagement Co
- Local Pub Grows Unique Community Ownership Program
- Heartfelt Dreams Foundation Launches Campaign to Build CHD Hospital