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Sensitive method to detect CD38 activity in a variety of sample types.
MILPITAS, Calif. - Californer -- Cluster of differentiation 38 (CD38), also known as cyclic ADP ribose hydrolase is a multifunctional enzyme that catalyzes the synthesis and hydrolysis of Nicotinamide Adenine Dinucleotide (NAD) to Nicotinamide and ADP-ribose (ADPR). Additionally, CD38 generates second messengers, cyclic ADP-ribose using NAD as the substrate. Due to its role in NAD+ metabolism, the study of CD38 and its functions has been of great importance. CD38 has been implicated in regulating NAD+ metabolism, aging, and other diseased conditions including obesity, diabetes, heart disease, asthma, inflammation etc. Furthermore, CD38 is expressed by lymphoid, myeloid, red blood cells and platelets, the highest expression being found in plasma cells. High expression of CD38 by plasma cells makes CD38 an ideal target for targeted immunotherapy. BioVision's CD38 Activity Assay Kit can be used to detect its activity in biological samples. The kit utilizes the ability of active CD38 to catalyze the conversion of a CD38 substrate to a fluorescent product (Ex/Em = 300/410 nm). This assay kit provides a rapid, simple and sensitive method to detect CD38 activity in a variety of sample types.
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Figure: (A) CD38 Standard Curve. (B) Reaction Kinetic curve of CD38 Positive Control, spleen lysate and B-cell lysate. (C) CD38 activity assayed in rat spleen lysate (2 μg) and B-cell lysate (2.7 μg). Assays were performed following the kit protocol.
For more information on this Assay Kit, visit: https://www.biovision.com/cd38-activity-assay-kit-fluorometric.html
www.biovision.com
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Figure: (A) CD38 Standard Curve. (B) Reaction Kinetic curve of CD38 Positive Control, spleen lysate and B-cell lysate. (C) CD38 activity assayed in rat spleen lysate (2 μg) and B-cell lysate (2.7 μg). Assays were performed following the kit protocol.
For more information on this Assay Kit, visit: https://www.biovision.com/cd38-activity-assay-kit-fluorometric.html
www.biovision.com
Source: BioVision Inc
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