Adenosine Monophosphate (AMP) Colorimetric Assay Kit
The Californer/10035300

MILPITAS, Calif. - Californer -- Adenosine Monophosphate (AMP), also known as 5'-adenylic acid, consists of adenine, ribose and phosphate. AMP can be produced during ATP synthesis by the enzyme adenylate kinase by combining two ADP molecules, as a result of the hydrolysis of ATP/ADP, or when RNA is broken down. AMP plays an important role in many cellular metabolic processes, such as Ca2+ signaling, cell migration, cytokine secretion etc. AMP is an activator of AMP-activated protein kinase, which regulates glucose uptake, fatty acid uptake, fatty acid oxidation etc. AMP levels can be measured by luciferase/luciferin mediated assays. However, luciferase signal is unstable and luminescent equipment is generally not available in most laboratories.
BioVision's AMP Colorimetric Assay Kit provides a convenient method to detect AMP in biological samples. In this assay, AMP is converted to pyruvate in the presence of Pyrophosphate and Phosphoenolpyruvate. This is followed by a set of enzymatic reactions to generate a colored product with a strong absorbance at 570 nm. The absorbance is proportional to the amount of AMP present in samples. The kit is rapid, sensitive, easy to use and high-throughput adaptable. It can measure AMP level lower than 10 µM in various sample types.

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Figure:  Measurement of AMP in different cell lysates: Jurkat (20 µg), HeLa (30 µg) and HEK293 (60 μg). Assays were performed following K229 protocol.

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Source: BioVision Inc
Filed Under: Biotech

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