Adenosine Monophosphate (AMP) Colorimetric Assay Kit
The Californer/10035300

MILPITAS, Calif. - Californer -- Adenosine Monophosphate (AMP), also known as 5'-adenylic acid, consists of adenine, ribose and phosphate. AMP can be produced during ATP synthesis by the enzyme adenylate kinase by combining two ADP molecules, as a result of the hydrolysis of ATP/ADP, or when RNA is broken down. AMP plays an important role in many cellular metabolic processes, such as Ca2+ signaling, cell migration, cytokine secretion etc. AMP is an activator of AMP-activated protein kinase, which regulates glucose uptake, fatty acid uptake, fatty acid oxidation etc. AMP levels can be measured by luciferase/luciferin mediated assays. However, luciferase signal is unstable and luminescent equipment is generally not available in most laboratories.

More on The Californer

• Historic 1947 Trumpy Yacht Mauretania Featured in Wooden Boat Magazine
• California Music Hall of Fame 2025 Induction Ceremony at Warner Bros. Studios November 15th
• Angel Light Academy Honors 8 Community Members Who Demonstrate Excellence in Leadership and Service 18th Annual Above & Beyond Awards November 8
• Culture, Classical Guitar and Comedy... HealthyLife.net Radio adds new programs
• THINKWARE to Showcase Three Next-Generation Dash Cams at SEMA Show

BioVision's AMP Colorimetric Assay Kit provides a convenient method to detect AMP in biological samples. In this assay, AMP is converted to pyruvate in the presence of Pyrophosphate and Phosphoenolpyruvate. This is followed by a set of enzymatic reactions to generate a colored product with a strong absorbance at 570 nm. The absorbance is proportional to the amount of AMP present in samples. The kit is rapid, sensitive, easy to use and high-throughput adaptable. It can measure AMP level lower than 10 µM in various sample types.

Figure:  Measurement of AMP in different cell lysates: Jurkat (20 µg), HeLa (30 µg) and HEK293 (60 μg). Assays were performed following K229 protocol.

For more info on this kit, visit: https://www.biovision.com/amp-colorimetric-assay-kit.html