Human Insulin Fibrillation Inhibitor Screening Kit (Fluorometric)
The Californer/10154544

For screening potential inhibitors of human insulin fibrillation.

MILPITAS, Calif. - Californer -- Human insulin consists of 51 amino acids, with a molecular mass of 5.81 kDa. It contains two peptide chains (A & B), which are linked by two disulfide bonds. Human insulin is widely used as a drug for treating diabetes patients. However, under certain conditions, human insulin aggregates into insoluble fibrils via fibrillogenesis, which leads to complications including reduced insulin potency, occlusion of insulin delivery devices, potentially increased immunological potential etc. Therefore screening of potential Inhibitors of human insulin fibrillation is critical for preventing insulin aggregation and developing potential therapeutics.

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BioVision's Human Insulin Fibrillation Inhibitor Screening kit, insulin fibrillation is induced by low pH and high temperature. Fibrillation Assay Probe then binds to the beta sheets of the insoluble fibrils to generate a strong fluorescence signal measured at Ex/Em = 440/500 nm. But in the presence of an insulin fibrillation inhibitor, fibrillation is inhibited thereby reducing the fluorescence signal. The assay is simple, reliable and is high-throughput adaptable.

Figure: Inhibition of Human Insulin Fibrillation by inhibitor (IC50 = 4 µM). Assay was performed following the kit protocol.

Storage Conditions -20ºC
USAGE For Research Use Only! Not For Use in Humans.
For more information on this Assay Kit Cat# K2104

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Source: BioVision Inc
Filed Under: Biotech

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