Pyruvate Carboxylase Activity Assay Kit (Colorimetric)
The Californer/10130656

MILPITAS, Calif. - Californer -- Pyruvate carboxylase (PC) catalyzes the irreversible carboxylation of pyruvate to oxaloacetate (OAA), depending on the species. It is a tetramer composed of four identical monomers each with a molecular weight of 125 kDa. OAA produced by PC is an important intermediate in numerous biological pathways. In mammals, PC plays an essential role in gluconeogenesis, lipogenesis, synthesis of neurotransmitters, and glucose-induced insulin secretion by pancreatic islets. During nutritional overload, PC levels are increased in the pancreatic β-cells to increase pyruvate cycling due to an increase in glucose levels. PC deficiency can lead to lactic acidosis due to the accumulation of lactic acid. BioVision's

Pyruvate Carboxylase Activity Assay Kit provides a facile, rapid way to measure PC activity in various biological samples. In this kit, pyruvate is carboxylated to OAA, which is further detected by the oxidation of NADH. Therefore, a decrease in NADH absorbance at 340 nm is proportional to the amount of PC in samples. The assay is simple, sensitive, and can detect PC activity lower than 200 μU in samples. Google Description: Pyruvate carboxylase (PC) catalyzes the irreversible carboxylation of pyruvate to oxaloacetate (OAA), depending on the species. It is a tetramer composed of four identical monomers each with a molecular weight of 125 kDa. OAA produced by PC is an important intermediate in numerous biological pathways. In mammals, PC plays an essential role in gluconeogenesis, lipogenesis, synthesis of neurotransmitters, and glucose-induced insulin secretion by pancreatic islets. During nutritional overload, PC levels are increased in the pancreatic β-cells to increase pyruvate cycling due to an increase in glucose levels. PC deficiency can lead to lactic acidosis due to the accumulation of lactic acid.

More on The Californer

• CGI Announces Pre-Order Launch for New Integrated Behavioral Health Book
• California leaders announce historic Veterans and Affordable Housing Bond Act of 2026 to expand homeownership and build affordable housing for generations of Californians
• Long Beach to Conduct Annual Summer Recess for City Council Meetings During June, July
• Governor Newsom announces $1.3 billion in new private investment, creating thousands of new jobs in key industries across California
• Looking for a Rodeo and Line Dance Rhinestone Belt? Western Fashion Fans Turn to TRB Belts

BioVision's Pyruvate Carboxylase Activity Assay Kit provides a facile, rapid way to measure PC activity in various biological samples. In this kit, pyruvate is carboxylated to OAA, which is further detected by the oxidation of NADH. Therefore, a decrease in NADH absorbance at 340 nm is proportional to the amount of PC in samples. The assay is simple, sensitive, and can detect PC activity lower than 200 μU in samples.

Figures: (a). NADH Standard Curve, results from multiple experiments. (b). Pyruvate Carboxylase activity of Positive Control. (c). PC activity in Rat Brain (2 μg protein) and 3T3 cells (2 μg protein) lysates. All assays were performed following kit protocol.

For more information on this Assay kit Cat# K2075, Visit: https://bit.ly/3gprZIq